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In the 1 week, the fresh lungs were wet which have cooler PBS to get rid of blood and you will flash frozen inside drinking water nitrogen. 5-15 milligrams away from flash-suspended lung tissue are incubated having 0.dos mM CMH inside the KHB that has one hundred μM inside the 2 hundred μL away from overall frequency for starters h during the 37° C. Supernatant is actually accumulated and placed in PTFE tube and you will work at from the 77 K in the hand dewar. (A) Nitroxide amount (μM away from nitroxide normalized to at least one milligrams away from tissue). (B) Representative spectra away from nitroxide inside lung muscle of PBS- and you may Bleo-addressed rats. (A) Raw 264.7 tissues stimulated with ten μM PMA and you may EPR spin probe, CMH 0.25 mM (fifty min at the 37 °C) that have (black) otherwise rather than (red) pretreatment with 30 You/mL SOD.
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